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    Structured Review

    PromoCell hydrocortisone
    Hydrocortisone, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+cell+growth+medium+mv+kit/Endothelial+Cell+Growth+Medium+MV+2+KIT/pmc13019081-439-15-16
    Average 96 stars, based on 121 article reviews
    hydrocortisone - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    In Vivo:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Recombinant:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    High Performance Liquid Chromatography:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Purification:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Permeability:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    In Vitro:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Membrane:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Saline:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Injection:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Labeling:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Standard Deviation:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Cell Culture:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Staining:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Expressing:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Western Blot:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Real-time Polymerase Chain Reaction:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Confocal Microscopy:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Double Staining:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Construct:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Activation Assay:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Fluorescence:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Immunofluorescence:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.

    Phospho-proteomics:

    Article Title: Human coronary artery tri-culture organ-chip recapitulates anti-inflammatory effect of pulsatile wall strain
    Article Snippet: PrePrint: n Coronary Artery Endothelial Cells (HCAECs) were purchased from Promocell (C-12221, derived from a single donor, N=1) and maintained in complete Endothelial Cell Growth Medium MV with 5% serum (v/v) (Promocell). This medium was supplied as a kit and consisted of Basal Medium (C-22220) and Supplement Mix (C-22120) with Endothelial Cell Growth Supplement (0.004 mL/ml), Epidermal Growth Factor (recom

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial-cadherin in endothelial cells.
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM-2 Bullet Kit (CC-3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C-22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma-free.

    Article Title: Porphyromonas gingivalis outer membrane vesicles increase vascular permeability by inducing stress fiber formation and degrading vascular endothelial‐cadherin in endothelial cells
    Article Snippet: Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).Human primary umbilical vein endothelial cells (HUVECs) (C2519A; Lonza Walkersville, MD, USA) were obtained from Lonza Walkersville and cultured using an EGMTM‐2 Bullet Kit (CC‐3126; Lonza Walkersville).. Human primary pulmonary microvascular endothelial cells (HPMECs) (C‐12281; PromoCell GmbH, Heidelberg, Germany) were purchased from PromoCell and maintained in MV medium prepared using an Endothelial Cell Growth Medium MV Kit (C‐22121; PromoCell).. HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.HUVECs and HPMECs used in the experiments were authenticated based on high expression of VEc protein and cell morphology and were verified to be mycoplasma‐free.



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